Session: 02-14-01: 3D/4D BioManufacturing, BioMaterials, & Computational Modeling
Paper Number: 95300
95300 - A Bio-Printing Strategy to Fabricate Geometrically Accurate 3d Scaffolds
Due to the inherent capability of releasing cell-seeded and cell-laden biocompatible materials in a predefined location to manufacture patient-specific tissue scaffolds, 3D bioprinting has gained popularity in recent times. Various natural and synthetic biopolymers can be released using a multi-nozzle extrusion-based 3D bioprinting system. The investigations of material-to-material and cell-to-material interactions can expose relevant information about the scaffold geometry, cell viability, and proliferation. Although the biocompatibility and high-water content distinguish natural hydrogels as a demanding candidate for bio-printing, the lack of mechanical integrity makes it challenging to get defined scaffold geometric fidelity.
Polycaprolactone (PCL) is a potential synthetic bioprinting material that can provide required mechanical integrity for fabricated scaffolds, such as bone, tracheal, other cartilages, and skin scaffolds. It is the most widely used 3D-printable biomaterial which was approved by the United States Food and Drug Administration (FDA) for internal use in humans. Even it has excellent biocompatibility, PCL is hydrophobic and unfavorable for cell attachment. Therefore, it is often used with other natural biopolymers such as alginate, GelMA, Collagen.
In this research, the printing process parameters such as print speed, extrusion pressure, bed and nozzle temperatures, nozzle size will be optimized to fabricate pure PCL with various molecular weights. Compressive stress for various pore sizes and geometries will be identified. Then, a strategy to fabricate scaffolds with natural and synthetic polymers will be demonstrated. Various natural polymers such as Alginate (A), Carboxymethyl Cellulose (CMC); TEMPO mediated Nano Fibrillated Cellulose (TO-NFC) will be used with a variable composition. The optimum compositions will be identified based on various rheological properties such as flow curves, Linear Viscosity Range (LVR), and recovery rate. The dependence of scaffold geometry on the rheological properties of natural polymers will also be identified. As part of mechanical properties, the yield and flow stress of the material compositions will be determined. The interactions between the natural and synthetic polymers of the fabricated scaffolds and consequently, geometric fidelity will be analyzed in terms of porosity, layer heights, and mechanical properties. To execute this, a set of samples including 1D filament, bi-layer scaffold with 2D uniform and variational pore geometries, and 3D regular and freeform scaffolds will be fabricated using the natural and synthetics polymers simultaneously. The morphological and numerical analysis will be conducted to determine the optimum strategy to fabricate hybrid scaffolds. The successful application of this research can assist in fabricating clinically relevant multi-material scaffolds with defined geometric structures.
Presenting Author: Connor Quigley Keene State College
Presenting Author Biography: He is a Junior student at the Department of Sustainable Product Design and Architecture. He is a self-motivated and talented research student. His interests are in digital design and manufacturing.
Authors:
Connor Quigley Keene State CollegeSlesha Tuladhar Keene State College
MD AHASAN HABIB Keene State College
A Bio-Printing Strategy to Fabricate Geometrically Accurate 3d Scaffolds
Paper Type
Technical Paper Publication