Session: 13-04-01: Applications of Micro and Nano Systems in Medicine and Biology

Paper Number: 145012

145012 - An Optimized Laser-Induced Graphene-Based Electrochemical Biosensor Modified With Graphene-Conductive Ink for Alpha-Fetoprotein Detection. 

Abstract

In this work, a label-free electrochemical immunosensor was fabricated for the detection of alpha-fetoprotein (AFP) based on Laser-Induced Graphene (LIG) and modified with developed graphene polyaniline (G-PANI) ink. Alpha-fetoprotein (AFP) is a prominent biomarker for the diagnosis of primary liver cancer. For the prevention and treatment of hepatocellular carcinoma (HCC), it is essential to ensure the accurate detection of AFP. The performance of our designed immunosensor was evaluated by differential pulse voltammetry (DPV) and Chronoamperometry (CA) techniques to detect cancer proteins in the phosphate buffer saline (PBS) and serum. Both electrochemical analyses in different concentrations of AFP resulted in a linear relationship between peak current and the logarithmic plot of the concentrations with a correlation coefficient, R² = 0.99. The limit of detection was also calculated to be ~0.73 ng/mL in the range of 4 ng/mL-500 ng/mL. The nearly identical current outputs in the DPV measurements were obtained consecutive ten times which represents the sensor’s stability with an RSD of 1.66%. Furthermore, the performance of the fabricated sensor in more complex biological environments was studied by detecting AFP in human serum (H4522). The current response was linearly proportional to the AFP concentration in serum samples. The results showed that the proposed immunosensor can be successfully used to detect AFP in PBS and undiluted human serum.

Presenting Author: Ridma Tabassum The University of Texas Rio Grande Valley

Presenting Author Biography: Ridma Tabassum is a graduate student in the Department of Electrical Engineering at the University of Texas Rio Grande Valley (UTRGV), USA. She is also working as a Graduate Research Assistant at UTRGV. She is deeply engaged in research involving graphene-based electrochemical sensors. One of the projects she is actively involved in is titled " Improvement of laser-induced graphene-based electrochemical biosensors through graphene-conductive polymer ink coating for sensitive detection of alpha-fetoprotein." This endeavor aims to create portable and cost-effective graphene-based biosensors for the detection of biomarkers associated with hepatocellular carcinoma (HCC).

Authors:

Ridma Tabassum The University of Texas Rio Grande Valley
Pritu Parna Sarkar The University of Texas Rio Grande Valley
Ahmed Jalal The University of Texas Rio Grande Valley
Ali Ashraf The University of Texas Rio Grande Valley
Nazmul Islam The University of Texas Rio Grande Valley