Session: Rising Stars of Mechanical Engineering Celebration & Showcase

Paper Number: 148001

148001 - Bone-Based Nanoparticles (Bnps) Promotes Osteogenic Differentiation of Mesenchymal Stem Cells (Mscs) 

Introduction: Bone related diseases such as osteoporosis, osteogenesis imperfecta, and Paget’s disease, are primarily treated with pharmacologic therapies that often exhibit limited efficacy and substantial side effects. Bone marrow derived mesenchymal stem cells (MSCs) play a key role in fracture repair and bone tissue engineering by differentiation to bone-forming osteoblasts and cartilage-forming chondrocytes. MSCs differentiation into osteoblasts is a major mechanism to repair damaged bone.  However, the precise mechanisms that control their plasticity remain poorly understood, especially how hMSCs can be differentiated into osteoblasts for bone formation. Enhancing osteogenesis regulation is crucial for improving the therapeutic efficacy of MSC-based bone tissue engineering. Therefore, recent efforts have concentrated on strategies to enhance MSC osteogenesis, which involve biophysical and biochemical stimulation.   

Methodology: We has recently developed a new type of NPs using the decellularized porcine bones (referred to as bone nanoparticles, BNPs), Figure 1A-1B. The BNPs are nano-sized, cell/antigen free, and protein rich. BNPs can enter cells to induce osteogenic differentiation of MSCs and can promote in vivo bone regeneration. We first evaluated the cell viability after BNPs incubation with MSCs for up to two weeks. We accessed the viability using live/dead assay and WST assay. To further investigate the effects of BNPs on MSCs osteogenic differentiation. We measured the early osteogenic differentiation marker RUNX2 and OCN expression after 2 weeks of osteogenic induction

Preliminary results and conclusions: 

Conclusions: We developed a novel BNPs from porcine bones and characterized the BNPs using SEM and TEM. Our results showed these novel BNPs are non-toxic and can be stable up to 1 year. Our results showed MSCs osteogenic differentiation was enhance by increasing the early differentiation marker RUNX2 and OCN. A significant increase of calcium deposition was observed when MSCs were co-cultured with BNPs. There results indicate that BNPs can be utilized for a novel biomaterials that could potentially enhance stem cell differentiation, which could provide valuable information for tissue repair and regenerative medicine.

Presenting Author: Shue Wang University of New Haven

Presenting Author Biography: Dr. Shue Wang is an Assistant Professor in the Department of Chemistry, Chemical and Biomedical Engineering at University of New Haven. She received her Ph.D. in mechanical engineering from the University of Arizona in 2015. After graduation, she worked at the University of Michigan as a postdoctoral researcher focus on synthetic biology. Her research focuses on biosensing, regenerative medicine, tissue engineering, single cell analysis, mechanobiology, lab-on-a-chip, synthetic biology, and applying micro-engineered tools to understand the complex biological systems at both the molecular and cellular levels. Dr. Wang received the NSF CAREER award in 2022.

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