Session: 06-05-02: Biomedical Devices, Sensors, and Actuators

Paper Number: 142655

142655 - Digital Micro-and Nanofluidic Systems for Infectious Diseases Sensing 

Accurate analysis of human papillomavirus (HPV)-infected cervical cancer is crucial for early diagnosis and treatment. In this study, we introduce a novel energy transfer-labeled oligonucleotide probe to enhance the loop-mediated isothermal amplification (LAMP) assay, improving the detection of HPV 16. Our method, conducted as a single-step assay within a digital nanofluidic chip with multiple reaction reservoirs, enables efficient target amplification under isothermal conditions. Targeting an HPV 16 gene, our chip detects HPV DNA at concentrations as low as 1 fM, covering a dynamic range of five orders of magnitude. Notably, our digital chip offers highly quantitative detection of target genes at low concentrations, with fluorescence signals correlating with target concentration in the microwells. Additionally, we've developed a computer vision system for automated and 100% accurate quantification of target concentrations. This research has promising applications in clinical diagnosis and can seamlessly integrate into hospital and point-of-care settings.

Presenting Author: Ke Du The University of California, Riverside

Presenting Author Biography: Dr. Ke Du is an assistant professor of chemical and environmental engineering at the University of California, Riverside and leads the Nanobiosensing, Nanomanufacturing, and Nanomaterials (3N) Lab. Additionally, He holds courtesy appointments in the department of bioengineering, materials science, and mechanical engineering.
Du’s research interests include in vitro molecular diagnostics, in vivo bioimaging, nanotoxicity, and nanomanufacturing. He is recipient of numerous awards and honors such as the EIPBN Best Journal Paper Award (2022), the NIH Maximizing Investigators’ Research Award (2021), the Burroughs Wellcome Fund (BWF) Collaborative Travel Grant (2019), and the James H. Potter Award for the outstanding Ph.D. students (2014).
He has been recognized as a global rising star in sensing by ACS Sensors and a finalist for the MINE 2020 Young Scientists Award. Du’s research has been supported by NIGMS, NIAID, NSF, USDA, DOE, BWF, the UNYTE Translational Research Network, and industry partners such as L3Harris, Mammoth Biosciences, Colgate Palmolive, and Biological Mimetics. He serves as an early career editorial advisory member for Biomicrofluidics (AIP Publishing) and Sensors and Actuators Reports (Elsevier).

Authors:

Li Liu The University of California, Riverside
Stephen Dollery Biological Mimetics Inc.
Gregory Tobin Biological Mimetics, Inc.
Guoyu Lu The University of Georgia
Ke Du The University of California, Riverside