Session: Research Posters

Paper Number: 115038

115038 - Analysis of Radiopaque Coatings of Pcl Flow Diverters for Brain Aneurysms 

Bioresorbable flow diverters (FDs) have been of great interest recently as an endovascular blood flow regulating device for curing brain aneurysms. Bioresorbable FDs are resorbed by the body when they are done with the complete occlusion of aneurysms and remodeling of the blood vessels network. Recently, our group developed non-braided bioresorbable polycaprolactone (PCL) FDs, which can be deployed through a vascular catheter delivery system. However, the visibility of PCL FDs under angiography has been a challenge. The visibility of FDs during and after placement is critical for ensuring proper deployment, wall apposition, patency of the side branches of the blood vessel, and complete occlusion of aneurysms. This poster presents the analysis of the three different radiopaque coatings and their effect on cell viability, functions, and cytotoxicity. The square PCL tile plates (8 mm by 8mm) were 3D printed from medical-grade PCL filament. The  BaSO4, Bi2O3, and tantalum powder were mixed with 0.4 g PCL in 4 ml acetone for 30 min using an automated sonication system for making three different coating pastes with concentrations of 0.25 g/ml, 0.2 g/ml, 0.15 g/ml and 0.1 g/ml for each of these coating materials. The visibility of the coated tiles was tested and analyzed with a hand-held x-ray machine and using ImageJ software. The coated samples were incubated with human umbilical vein endothelial cells (HUVEC) for 48h to evaluate cell proliferation and adhesion.   The PCL FDs were then covered with 1 mm wide rings at the two ends and one in the middle with 0.2 g/ml and 0.15 g/ml concentration of BaSO4, Bi2O3 and tantalum powder paste. The deployability was demonstrated through a 4 Fr catheter in a PDMS aneurysm model. The visibility of the PCL FDs was imaged using the hand-held x-ray machine. The intensity was estimated using ImageJ software. The results showed that the higher the concentration of the coating materials, the better visibility under x-ray was observed regardless of the coating materials. However, Bi2O3 was more visible with higher intensity among the three coating materials. HUVEC cell proliferation and adhesion on the Bi2O3-coated PCL tiles were the highest in 0.15 g/ml concentration samples. Lactate dehydrogenase (LDH) release showed that all radiopaque-coated PCL tiles were non-toxic regardless of the coating materials. The differences in LDH results among the coated and uncoated samples with three different coatings were not statistically significant. Results also showed that the radiopaque coatings on PCL FDs reduced the flexibility of the PCL FDs. However, 0.15 g/ml coating of Bi2O3 and BaSO4 on PCL FDs was flexible enough for loading and unloading from 4 Fr catheter into the aneurysm model. The overall results regarding x-ray visibility, HUVEC cell functions, and deployability of the developed  bioresorbable PCL FDs were promising.

Presenting Author: Noor Akour University of Central Oklahoma

Presenting Author Biography: Undergraduate research assistant

Authors:

Mohammad Hossan Univ Of Central Oklahoma
Noor Akour University of Central Oklahoma
Alex Matsayko University of Central Oklahoma
Melville Vaughan University of Central Oklahoma