Session: Research Posters

Paper Number: 165478

Graphene Screen-Printed Electrodes for Wound Biosensing 

The monitoring of wound healing is critical to improve patient outcomes, especially for the chronic wounds, which often have complications like delayed healing and infections. Current methods for monitoring wound healing are invasive and costly. Also, these methods have a limited ability to provide real-time, continuous data. This study seeks to develop a graphene screen-printed electrodes (SPEs) electrochemical biosensor that is entirely non-invasive and efficient to constantly monitor wound healing in real time. This research study focuses specifically on electrochemical detection of the Human Neutrophil Elastase (HNE) which is one of the most abundant neutral proteinases in chronic wounds. Studies have found that Human Neutrophil Elastase (HNE) activity is elevated in chronic wounds, while diminished in healing wounds. By investigating the electrochemical responses of Human Neutrophil Elastase (HNE), could lead to a possible marker regarding severe wound inflammation or infection.

The INTELLO electrochemical biosensing device will be used in this study for biomarker response analysis, along with type 110 carbon screen-printed electrodes (Aux.:C; Ref.:Ag) from Metrohm. The electrochemical performance will be assessed using different Human Neutrophil Elastase (HNE) concentrations to determine the sensor's sensitivity, selectivity, and dynamic range of detection. The concentration of Human Neutrophil Elastase (HNE) will be quantified through the use of the HNE ELISA kit (antibody and antigen pairs) from Abcam, and Phosphate Buffered Saline (PBS, pH 7.2) will be used as the buffer solution for this research study.

This research seeks to evaluate the potential and effectiveness of graphene screen-printed electrodes biosensor to detect Human Neutrophil elastase (HNE) in real-time and to determine whether the device can be used to continuously monitor wound healing. If successful, the approach will create a biosensor for rapid, continuous, and non-invasive monitoring of wound inflammation and tissue degradation, allowing healthcare providers to provide rapid and effective patient care decisions. In addition, this method can reduce the healthcare costs compared to the traditional wound diagnostic methods.

The anticipated outcome of this study includes the validation of a graphene-based biosensor capable of detecting the Human Neutrophil Elastase (HNE) molecule in testing solution and, eventually, in the real wound samples. The results of this research could establish a way to integrate graphene-based biosensors into clinical settings. This would represent a novel and powerful method for treating wounds. Further development of the technology could transform wound care by offering real-time, point-of-care diagnostics, which can potentially guide timely interventions, increasing patient outcomes, and accelerating wound healing.

Presenting Author: Chanyapat Sriviroch University of South Florida

Presenting Author Biography: My name is Chanyapat Sriviroch. I'm an international student from Thailand, I completed my undergraduate degree in Mechanical Engineering at the University of South Florida and am currently pursuing my master’s in the same field. I recently joined the NSF semiconductor research traineeship program at USF. Additionally, I'm working on a research in micro-nano materials field.

In my career, I’ve worked as an HVAC Mechanical Engineer at a local engineering firm in downtown Tampa. During my time there, I had the opportunity to collaborate with many engineers, architects, clients and project managers, which gave me valuable insights into the dynamics of engineering projects and management skills.

Authors:

Chanyapat Sriviroch University of South Florida
Ali Ashraf University of South Florida
Ashok Kumar University of South Florida
A K M Abirul Haque University of South Florida